TMA by the Automated Tissue Microarrayer(Quick Ray Master)

1. Place the reference slide and the donor block on the microscope stage, and mark the position where you intend to extract the sample tissue with an oil pen.

2. Place donor blocks and recipient blocks on the block holders in the instrument

3. Quick Ray Master extracts the marked tissue from the donor block and delivers the extracted tissue into the correspondent holes of the pre-made recipient block (UB06)

4. Put the completed recipient block into the base mold with the face to be sectioned down and place it in an oven at 70 degree celsius for 30 to 60 minutes until the block is completely transparent, then take it out from the oven.

5. Place an embedding cassette on the top of transparent block and dispense liquid paraffin into the base mold adequately covering the cassette.
6. Solidify the block in a cold plate.

7. Sectioning by a microtome.   
8. Follow the next workflow